TETRANITRO-BLUE TETRAZOLIUM REDUCTION IN BACILLUS SUBTILIS
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چکیده
منابع مشابه
Tetranitro-blue Tetrazolium Reduction in Bacillus Subtilis
In order to decide whether in the bacterial cell the enzymes of the respiratory chain are located in "particulate elements" (1) or in the plasma membrane (2) we investigated the reduction of potassium tellurite in Gram-positive and Gram-negative bacteria. For the first time in 1962 (3), and in more detail in 1964 (4), we reported that with Bacillus subtilis we had found that reduction products ...
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In Bacillus subtilis the reduction of potassium tellurite and of tetranitro-blue tetrazolium (TNBT) give comparable results: reduced products appear deposited in particular membranous organelles. For this reason these specialized organelles are considered to be chondrioids (1-3). In the Gram-negative Proteus vulgaris similar membranous structures were never observed by us (4, 5). Therefore it i...
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Activity of the succinic dehydrogenase system was studied in Bacillus subtilis utilizing combined techniques of cytochemistry and electron microscopy. Organisms were incubated in a medium containing tetranitro-blue tetrazolium (TNBT) which served as an electron acceptor. Enzymatic activity, as evidenced by deposition of TNBT-formazan, was found on membranous organelles associated with the cytop...
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Succinic dehydrogenase (SDH) was localized in the mitochondria of Sarcoma 37 ascites tumor cells by the use of tetranitro-BT (TNBT) and nitro-BT (NBT) in smear preparations. Results with each tetrazolium salt as electron acceptor were evaluated with respect to: (a) size and shape of the formazan precipitate relative to standard mitochondrial morphology; (b) crystallization phenomena of reduced ...
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The emerging field of synthetic genomics is expected to facilitate the generation of microorganisms with the potential to achieve a sustainable society. One approach towards this goal is the reduction of microbial genomes by rationally designed deletions to create simplified cells with predictable behavior that act as a platform to build in various genetic systems for specific purposes. We repo...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1965
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.27.1.237